Abstract
IN at least two reports the method of sterilization for medium to be used for tissue culture experiments was autoclaving1,2. The question of the heat stability of gibberellin is raised. It is generally thought that gibberellin is stable on the basic side of neutrality and at room temperature at neutral or near neutral pH, and more labile at elevated temperatures at extreme pH values. It has been stated that even weakly acid media will destroy gibberellin and that it is much more stable in neutral and basic solutions (Stowe, B. B., private communication, November 7, 1958).
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References
Schroeder, C. A., and Spector, C., Science, 126, 701 (1957).
Henderson, James H. M., Nature, 182, 880 (1958).
Koch, F. G., and Hanke, M. E., “Practical Methods in Biochemistry”, 99 (Williams and Williams, 1943).
Phinney, Bernard O., Proc. U.S. Nat. Acad. Sci., 42, 185 (1956).
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HENDERSON, J. Influence of Hydrogen Ion Concentration and Autoclaving on Gibberellin. Nature 185, 628–629 (1960). https://doi.org/10.1038/185628a0
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DOI: https://doi.org/10.1038/185628a0
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