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Survival of the Twig of Woody Plants at −196° C.


I HAVE succeeded1 in keeping alive living materials from the cell interiors of which all the easily freezable water was drawn by sufficient extra-cellular freezing at −30° C., after immersion in liquid nitrogen.

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  1. 1

    Sakai, A., Low Temp. Sci., Ser. B, 14, 17 (1956).

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  2. 2

    Sakai, A., Low Temp. Sci., Ser. B, 16, 41 (1958).

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  3. 3

    Asahina, E., and Aoki, K., Nature, 182, 327 (1958).

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    Asahina, E., Low Temp. Sci., Ser. B, 16, 65 (1958).

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    Asahina, E. (unpublished).

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    Nei, T. (unpublished).

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SAKAI, A. Survival of the Twig of Woody Plants at −196° C.. Nature 185, 393–394 (1960).

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