Ribonucleases of Mouse Tissues and of the Ehrlich Ascites Tumour

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Abstract

DURING investigations of the infectivity for the Ehrlich mouse ascites tumour of ribonucleic acid isolated from tissues infected with Mengo encephalitis virus1, it was found that some factor in the ascitic plasma, the fluid in which the tumour cells are suspended in vivo, prevented the production of virus by the tumour cells. Since ribonuclease is known to destroy the infectivity of ‘Mengo-RNA’ preparations, the ascitic plasma was examined for the presence of this enzyme. It was found to have a significant level of activity, releasing 600 ± 90 µgm. ribonucleic acid-phosphorus/hr./ml. (average of 8 determinations) from 1 3 per cent ribonucleic acid at 37°C. and pH 7.4 (veronal-acetate buffer).

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References

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    Colter, J. S., Bird, H. H., Moyer, A. W., and Brown, R. A., Virology, 4, 522 (1957).

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ELLEM, K., COLTER, J. & KUHN, J. Ribonucleases of Mouse Tissues and of the Ehrlich Ascites Tumour. Nature 184, 984–985 (1959) doi:10.1038/184984a0

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