A Paper-Chromatographic Method for the Determination of Suitable Buffer Systems for Countercurrent Distribution

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Abstract

BETINA recently described1 a method for the determination of suitable pH values for the extraction of antibiotics. He used air-dried buffered paper, which is absorbent and tends to lead to the formation of ‘comets’, and he did not take into account the ratio of the volumes of the moving and stationary phases, r, which also influences the R F value. Thus the accuracy of his method is rather limited. Using a relatively moist paper and taking into account some quantitative relationships involved, it is possible to make this method more precise.

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References

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WAKSMUNDZKI, A., SOCZEWIŃSKI, E. A Paper-Chromatographic Method for the Determination of Suitable Buffer Systems for Countercurrent Distribution. Nature 184, 977–978 (1959) doi:10.1038/184977b0

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