Abstract
IN a screening programme for antibiotic effects of Basidiomycetes we found a strain which inhibited Candida albicans. The antifungal substance was produced by this strain in surface and submerged culture in a medium containing peptone, glucose, inorganic salts and aneurin. The substance for which we provisionally propose the name ‘antibiotic-T’ was easily extracted from the fermentation liquor by most of the usual solvents. After extraction with benzene and evaporation of the solvent the residue became crystalline within a few days. Recrystallization twice from methyl alcohol gave pure crystals (prisms), melting point 126°C.,[α]D 20 + 13.5°, c. 1 in chloroform. No ultra-violet absorption characteristic of antifungal antibiotics of the polyene type was found. The antibiotic contains no halogen, sulphur or nitrogen. Micro-analyses: found (per cent): carbon, 68.51; hydrogen, 7.6; and oxygen, 25.0. The substance is very slightly soluble in hot water and gives a neutral solution; it is soluble in alcohols and sunflower oil and readily soluble in non-polar solvents. Its solution in water is stable for two months, is thermo-stable at its boiling point, but is inactivated at pH 12 within a few hours.
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References
Freeman, G. G., and Gill, J. E., Nature, 166, 698 (1950).
Freeman, G. G., J. Gen. Microbiol., 12, 213 (1955).
Freeman, G. G., Gill, J. E., and Waring, W. S., J. Chem. Soc., 1105 (1959).
Yoshii, H., Ann. Phytopath. Soc. Japan, 14, 84 (1950).
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GLAZ, E., SCHEIBER, E., GYIMESI, J. et al. A New Trichothecin-like Antifungal Antibiotic. Nature 184, 908 (1959). https://doi.org/10.1038/184908a0
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DOI: https://doi.org/10.1038/184908a0
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