Occurrence of 3-Indolylacetic and 3-Indolecarboxylic Acids in Tomato Crown—Gall Tissue Extracts

Article metrics


A NUMBER of publications1 report high auxin activity in extracts of tumour tissue initiated by the crown-gall organism, Agrobacterium tumefaciens, and indirect evidence indicates a high rate of auxin metabolism in this tissue2. Suggestions have been made that 3-indolylacetic acid is involved, although this has not been proved. There is evidence that auxin activity occurs at the same or a similar R F as 3-indolylacetic acid on one-dimensional chromatograms and other investigations indicate that 3-indolylacetic acid or “an auxin with same R F” is present in extracts of aseptically cultured gall tissue of tobacco and sunflower3. We have carried out single and two-dimensional paper chromatography with the acid fraction from ether extracts of large quantities of tomato crown-gall tissue grown on the stems of whole plants to determine whether 3-indolylacetic acid is in fact present.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Link, G. K. K., and Eggers, V., Bot. Gaz., 103, 87 (1941). Henderson J. H., and Bonner, J., Amer. J. Bot., 39, 444 (1952). Kulescha, Z., Année biol., 30, 319 (1954).

  2. 2

    Locke, S. B., Riker, A. J., and Duggar, B. M., J. Agric. Res., 57, 21 (1938).

  3. 3

    Bitancourt, A. A., Année biol., 30, 361 (1954). Schwarz, K. R., Dierberger, R., and Bitancourt, A. A., Arch. Biol., S. Paulo, 22, 93 (1955).

  4. 4

    Fawcett, C. H., Pascal, R. M., Pybus, M. B., Taylor, H. F., Wain, R. L., and Wightman, F., Proc. Roy. Soc., B, 150, 95 (1959).

  5. 5

    Kaper, J. M., and Veldstra, H., Biochim. Biophys. Acta, 30, 401 (1958). Schwarz, K., and Bitancourt, A. A., Science, 126, 607 (1957).

  6. 6

    Booth, A., J. Exp. Bot., 9, 306 (1958).

  7. 7

    Nichols, R., Nature, 181, 919 (1958).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

CLARKE, G., DYE, M. & WAIN, R. Occurrence of 3-Indolylacetic and 3-Indolecarboxylic Acids in Tomato Crown—Gall Tissue Extracts. Nature 184, 825–826 (1959) doi:10.1038/184825a0

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.