An Attenuated Strain of Canine Distemper Virus in Tissue Culture

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CANINE distemper virus was cultivated in dog kidney tissue culture with a clear cytopathogenic effect1 and the 56th passage of the virus was tested in ferrets. Each of four non-immune ferrets were inoculated intraperitoneally with 1 ml. of tissue culture fluid (105.5 TCID 50). Three non-immune ferrets were kept in the same laboratory as control animals and a further two in a neighbouring room in order to test for possible air-borne contamination from the inoculated animals. During an observation period of 25 days no animal showed any signs of disease. Blood was drawn from all the animals before the experiment and 17 days after inoculation. Tissue culture neutralization tests were performed with the sera, inactivated for half an hour at 56°C., against 300 TCID 50 of canine distemper virus after incubation at room temperature for one hour. The 50 per cent neutralizing titre of sera from inoculated animals was more than 10−2 (final dilution of serum) on day 17, while neutralizing antibodies were not found, either in the pre-inoculation sera or in the sera from the control animals on day 17.

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  1. 1

    Rockborn, G., Arch. Virusforsch., 8, 485 (1958).

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ROCKBORN, G. An Attenuated Strain of Canine Distemper Virus in Tissue Culture. Nature 184, 822 (1959) doi:10.1038/184822a0

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