Abstract
IT has recently been shown by several authors1–5 that plant tissue cultures grown in liquid media are composed of a population of single cells and small cell clusters. Such cultures represent an excellent source of large numbers of single cells and would be very useful for several types of experiments if the single cells could easily be isolated and grown.
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References
Steward, F. C., and Shantz, E. M., in “The Chemistry and Mode of Action of Plant Growth Substances”, edit. by Wain, R. L., and Wightman, F. (Butterworths Scientific Publications, London., 1955).
Nickell, L. G., Proc. U.S. Nat. Acad. Sci., 42, 848 (1956).
Muir, W. H., Hildebrandt, A. C., and Riker, A. J., Amer. J. Bot., 45, 589 (1958).
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White, P. R., “The Cultivation of Animal and Plant Cells” (The Ronald Press Co., New York, 1954).
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Torrey, J. G., Proc. U.S. Nat. Acad. Sci., 43, 887 (1957).
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BERGMANN, L. A New Technique for Isolating and Cloning Cells of Higher Plants. Nature 184, 648–649 (1959). https://doi.org/10.1038/184648a0
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DOI: https://doi.org/10.1038/184648a0
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