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Direct Spectroscopic Examination of Electrophoretic Zones in Agar Gel


ELECTROPHORESIS in agar gel, as described by Robinson et al. 1, has been found to be a valuable analytical method in the study of hæmoglobin variants. We are now able to extend its scope by carrying out spectroscopic examination of the separate zones directly without extraction from the gel. The value of this technique lies, in particular, in the fact that agar electrophoresis can be carried out with minute samples, and also that components present in proportions too small to make their isolation feasible can be examined. At the same time, the difficulty of extracting proteins from the gel is circumvented.

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