IN the course of the isolation of aldosterone from adrenal gland extracts (‘Eucortone’, Allen and Hanbury), the English authors detected a compound with properties similar to aldosterone in certain paper and column partition systems. This compound had ultra-violet absorption peaks at about 230 mµ and 280 mµ, and gave a positive reaction with the FeCl3–K3Fe(CN)6reagent. It therefore seemed probable that it was phenolic1. It also gave a blue fluorescence on irradiation with ultra-violet (compound X)2 and reduced blue tetrazolium at about the same rate as steroids having an α-ketol side chain. The compound ran as though slightly more polar than aldosterone in the Bush B5 paper system3 and could be completely separated from the steroid by column chromatography using the same solvent system2.
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Euw, v. J., Neher, R., Reichstein, T., Tait, J. F., Tait, S. A. S. and Wettstein, A. (Helv. Chim, Acta in the press).
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VON Euw, J., REICHSTEIN, T., NEHER, R. et al. 3,4,ω-Trihydroxyacetophenone 3-methyl Ether in Adrenal Extracts. Nature 184, 268 (1959). https://doi.org/10.1038/184268b0
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