Abstract
IT has been shown by Walker1 that in a mouse-liver homogenate in isotonic sucrose solution by far the greater part of the β-glucuronidase activity sediments in the cytoplasmic granules (mitochondria and microsomes). It was concluded that little, if any, of the enzyme is present in the nuclei or free in the cytoplasm. This was also true of rapidly growing or regenerating liver. Within the granules the enzyme displayed only fractional activity due to its inaccessibility to substrate. In these respects β-glucuronidase, in rat liver at least, resembles a number of hydrolytic enzymes, which, it has been suggested, are enclosed together within a special type of subcellular particle, the lysosome2.
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CONCHIE, J., LEVVY, G. Localization of β-Glucuronidase in Normal and Cancer Cells. Nature 184, 1709 (1959). https://doi.org/10.1038/1841709a0
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DOI: https://doi.org/10.1038/1841709a0
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