Abstract
SUCCESSFUL separations of amines by paper electrophoresis were first described by R. Weber1 who applied a potential gradient of 10 V./cm. at pH 3.8. Several authors2 have since reported on the usefulness of the technique for resolving mixtures of simple, biogenic and poly-amines. All, except J. Gayer3, have used low to moderate potential gradients and varying electrolyte solutions.
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References
Weber, R., Helv. Chim. Acta, 34, 2031 (1951).
Honegger, C. G., Helv. Chim. Acta, 39, 1671 (1956). Edwards, J. T., Chem. and Ind., 1274 (1956). Fischer, F. G., and Bohn, H., Z. physiol. Chem., 308, 108 (1957); Ann. Chem., 603, 232 (1957). Anet, E. F. L., Chem. and Ind., 1438 (1958). Herbst, E. J., Keister, D. L., and Weaver, R. H., Arch. Biochem. Biophys., 75, 178 (1958).
Gayer, J., Biochem. Z., 328, 39 (1956).
Bell, D. J., McIndoe, W. M., and Gross, D., Biochem. J, 71, 355 (1959)
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GROSS, D. High-Voltage Paper Electrophoresis of Aliphatic Amines and Related Compounds. Nature 184, 1633–1634 (1959). https://doi.org/10.1038/1841633a0
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DOI: https://doi.org/10.1038/1841633a0
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