Abstract
ONE of the most difficult problems in the culture of plant embryos is to find a satisfactory way of extracting the embryo from the seed without damage and in such a manner that both embryo and culture medium remain sterile. This is particularly difficult if the seed coat is very hard, and usually dissection under sterile conditions is the standard method employed1,2. The following technique was originally evolved to provide an easy method for extraction of mature Pæonia embryos from their seeds, and has since been used in the removal of embryos from other hard-coated seeds.
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References
Lofland, H. B., Bot. Gaz., 111, 307 (1950).
Rietsema, J., Satina, S., and Blakeslee, A. F., Amer. J. Bot., 40, 538 (1953).
Conard, H. S., Publ. Carneg. Inst., 4 (1905).
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GWYNNE, M. A Quick Method for Extracting Plant Embyros from Certain Types of Seed. Nature 184, 1588–1589 (1959). https://doi.org/10.1038/1841588b0
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DOI: https://doi.org/10.1038/1841588b0
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