Letter | Published:

Sectioning Large Heavily Sclerotized Whole Insects

Abstract

THE following account shows how to obtain much-improved sections of large heavily sclerotized whole insects. The method should be applicable to many other kinds of animals which present similar problems. Use is made of a fixing, dehydrating, and clearing agent, tetrahydrofuran, which produces a minimum of tissue hardening. It is highly volatile thus permitting rapid removal in the paraffin oven, dissolves paraffin and celloidin. Tetrahydrofuran (furan) has been used by other workers with good results1,2,3. Paraffin infiltration is done in a partial vacuum. The blocks may be soaked 24 hr. or longer in either ‘Mollifex’ (B.D.H.) or in water (preferably with a small amount of detergent) or in 75 per cent alcohol. Before sectioning, the blocks in the soaking medium, if such is used, are cooled to approximately 15°C. Better than average sections are obtained.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Dahme, E., and Rothemund, I., Zentralbl. allg. Path., 94, 14 (1955).

  2. 2

    Haust, M. Daria, Lab. Investig., 7, 58 (1958).

  3. 3

    Salthouse, T. N., Can. Ent., 90, 555 (1958).

  4. 4

    Palmgren, Axel, Nature, 174, 46 (1954).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.