Letter | Published:

8-Hydroxyquinaldic Acid: the Red Fluorescent Substance in the Urine of Pyridoxine-Deficient Rats


DURING paper chromatographic examination of urine from pyridoxine-deficient rats, an orange-red fluorescent spot was observed when the chromatograms were examined with ultra-violet light having a predominant wave-length of 2540 A. The physical and chemical properties of the compound were found to be different from xanthurenic acid, its conjugates1,2 or any of the well-known metabolites of tryptophan which were excreted in increased amounts during pyridoxine deficiency3. However, the fluorescence of the compound was very similar to 8-hydroxyquinaldic acid, which was found in urine of normal rabbits4 or in rat urine after the administration of xanthurenic acid5. Hence, an attempt was made to isolate and identify the compound.

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