Letter | Published:

Paper Electrophoretic Patterns obtained by Paraffin-Oil Fixation


DURING experiments aimed at improving present staining techniques of paper electrophoretic patterns, it was observed that paraffin-oil is strongly adsorbed to heat-denatured proteins giving rise to water-repellent protein-dye complexes. This permitted a method to be devised by which the total area occupied by each fraction of a protein mixture, such as serum, is visualized as a sharply delimited zone against a fully white background1.

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  1. 1

    Waldmann-Meyer, H., Seventh Colloqu. St. Jans Hosp. Brugge, May, 1959 (preliminary report).

  2. 2

    Freund, J., Amer. J. Clin. Path., 21, 645 (1951).

  3. 3

    Schilling, K., and Waldmann-Meyer, H., Acta Chem. Scand., 13, 1 (1959).

  4. 4

    Waldmann-Meyer, H., and Schilling, K., ibid., 13, 13 (1959).

  5. 5

    Wunderly, C., in “Electrophoresis” (Academic Press, New York, 1959).

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