Letter | Published:

Gel Filtration as a Method for Purification of Protein-bound Peptides Exemplified by Oxytocin and Vasopressin


WE would like to call attention to a new procedure suitable for purification of simple compounds weakly associated with high molecular weight substances. The method, which was proposed elsewhere by one of us1, is based on separation according to molecular size achievable by means of filtration through dextran gels2. The principle which involves three operations may be illustrated for protein-bound peptides by the following scheme :

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Porath, J., Biochim. Biophys. Acta (in the press).

  2. 2

    Porath, J., and Flodin, P., Nature, 183, 1657 (1959).

  3. 3

    Moore, S., and Stein, W. H., J. Biol. Chem., 211, 907 (1954).

  4. 4

    Dekanski, J., Brit. J. Pharmacol., 7, 567 (1952).

  5. 5

    Coon, J. M., Arch. internat. pharmacody. thérap., 62, 79 (1939).

  6. 6

    Lowry, O. L., et al., Biol. Chem., 193, 265 (1951).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.