Letter | Published:

Estimation of Vitamin C by Agar Diffusion

Abstract

THE assay of antibiotics by diffusion through agar is well known and was first described by Heatley1. Dingle et al. 2 determined the concentration of enzymes by mixing the substrate in agar and measuring the zone of reaction after applying paper disks saturated with enzyme solutions to the agar plate. In both of these methods the diffusion through the agar was proportional to the concentration of the solution applied.

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References

  1. 1

    Heatley, N. G., Biochem. J., 38, 61 (1944).

  2. 2

    Dingle, J., Reid, W. W., Solomons, G. L., J. Sci. Food Agr., 4, 149 (1953).

  3. 3

    Bessey, O. A., and King, C. G., J. Biol. Chem., 103, 687 (1933).

  4. 4

    Council on Foods of the Amer. Med. Assoc. (1939).

  5. 5

    Gawron, O., and Berg, R., Ind. Eng. Chem. (Anal. Ed.), 16, 757 (1944).

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