Letter | Published:

Estimation of Vitamin C by Agar Diffusion


THE assay of antibiotics by diffusion through agar is well known and was first described by Heatley1. Dingle et al. 2 determined the concentration of enzymes by mixing the substrate in agar and measuring the zone of reaction after applying paper disks saturated with enzyme solutions to the agar plate. In both of these methods the diffusion through the agar was proportional to the concentration of the solution applied.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Heatley, N. G., Biochem. J., 38, 61 (1944).

  2. 2

    Dingle, J., Reid, W. W., Solomons, G. L., J. Sci. Food Agr., 4, 149 (1953).

  3. 3

    Bessey, O. A., and King, C. G., J. Biol. Chem., 103, 687 (1933).

  4. 4

    Council on Foods of the Amer. Med. Assoc. (1939).

  5. 5

    Gawron, O., and Berg, R., Ind. Eng. Chem. (Anal. Ed.), 16, 757 (1944).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.