Prefreezing as a Method enabling Animals to survive Freezing at an Extremely Low Temperature

Article metrics

Abstract

IN a previous paper it was demonstrated that, after sufficient extracellular freezing, some intact Insects could survive freezing in liquid oxygen without any antifreeze agent1. This might be a method for keeping an entire organism alive at extremely low temperatures, provided that it is sufficiently frost-resistant. Some work along these lines has been carried out in our laboratory. In plant tissues, Sakai has already successfully applied the method to various hard woods2. In animal material it was examined with regard to various intact animals and to excised tissues or cells.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Asahina, E., and Aoki, K., Nature, 182, 327 (1958).

  2. 2

    Sakai, A., Low Temp. Sci., B, 14, 17 (1956).

  3. 3

    Asahina, E., ibid., B, 16, 65 (1958).

  4. 4

    Asahina, E., and Aoki, K., ibid., B, 16, 55 (1958).

  5. 5

    Asahina, E., Contr. Inst. Low Temp. Sci., 10, 83 (1956).

  6. 6

    Scholander, P. F., Flagg, W., Hock, R. J., and Irving, R., J. Cell. Comp. Physiol., 42, Suppl. 1, 1 (1953).

  7. 7

    Salt, R. W., Canad. J. Zool., 33, 391 (1955).

  8. 8

    Shinozaki, J., Zool. Mag. (Tokyo), 67, 38 (1958).

  9. 9

    Sakai, A., Low Temp. Sci., B, 16, 41 (1958).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

ASAHINA, E. Prefreezing as a Method enabling Animals to survive Freezing at an Extremely Low Temperature. Nature 184, 1003–1004 (1959) doi:10.1038/1841003b0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.