Isolation of Tobacco Leaf Cells Capable of Supporting Virus Multiplication

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BECAUSE of its waxy cuticle, plant leaf tissue admits few substances applied to the leaf surface. Biochemical studies on the effects of exogenous additives on cell metabolism may require such substances to enter the cells with a minimum delay between application and absorption. Work in this laboratory on the multiplication of tobacco mosaic virus in tobacco leaf tissue has prompted the development of a technique for the preparation of quantities of isolated tobacco leaf cells. These cells support virus multiplication and overcome the barrier to penetration of low molecular weight substances, presented either by the intact leaf or the detached leaf or leaf piece.

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  1. 1

    Chayen, J., Nature, 170, 1070 (1952).

  2. 2

    Stephenson, M. L., Thimann, K. V., and Zamecnik, P. C., Arch. Biochem. Biophys., 65, 194 (1956).

  3. 3

    Boardman, N. K., and Zaitlin, M., Virology, 6, 758 (1958).

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ZAITLIN, M. Isolation of Tobacco Leaf Cells Capable of Supporting Virus Multiplication. Nature 184, 1002–1003 (1959) doi:10.1038/1841002a0

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