Abstract
ESTIMATION of microquantities of purines by paper chromatography was first suggested by Hotchkiss1. Later, detection by photographing with an ultra-violet source has amplified the method2. Tennent et al. 3 and others have designed adaptors for the Beckman spectrophotometer to enable direct estimation of the purines from paper chromatograms. This method requires costly instrumentation, and is time-consuming unless an automatic recorder is provided.
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References
Hotchkiss, R. D., J. Biol. Chem., 175, 315 (1948).
Markham, R., and Smith, J. D., Biochem. J., 45, 294 (1949).
Tennent, D. M., Whitla, J. B., and Florey, K., Anal. Chem., 23, 1749 (1951).
Fisher, R. B., Parsons, D. S., and Morrison, G. A., Nature, 161, 764 (1948).
Hanes, C. S., and Isherwood, F. A., Nature, 164, 1107 (1949).
Briner, G. P., J. Chromatography (in the press).
Drake, N. A., Haines, W. J., Knoff, R. E., and Nielson, E. D., Anal. Chem., 28, 2036 (1956).
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JOHNSTONE, B., BRINER, G. Quantitative Estimation of Purines by the Visual Scanning of Photographs of Paper Chromatograms. Nature 183, 681–682 (1959). https://doi.org/10.1038/183681b0
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DOI: https://doi.org/10.1038/183681b0
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