Abstract
THE cultivation of multicellular marine algae under laboratory conditions has always proved to be a matter of difficulty. Most species die after a few months even if cultivated in seawater or enriched seawater. Henkel1 succeeded in cultivating Bangia pumila through several generations in an artificial medium, but she did not work with pure cultures. Koch2 produced an axenical culture of Porphyridium cruentum. His medium was not, however, chemically defined since it contained natural water.
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References
Henkel, R., Kieler Meeresforschungen, 8, 192 (1951).
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Provasoli, L., McLaughlin, J. J. A., and Droop, M. R., Arch. Mikroboil., 25, 392 (1957).
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Pringsheim, E. E., “Pure Cultures of Algae” (Cambridge, 1949).
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Holm-Hansen, O., Gerloff, G. C., and Skoog, F., Physiol. Plantar., 7, 665 (1954).
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Pintner, I. J., and Provasoli, L., J. Gen. Microbiol., 18, 190 (1958).
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FRIES, L. Goniotrichum elegans: a Marine Red Alga requiring Vitamin B12 . Nature 183, 558–559 (1959). https://doi.org/10.1038/183558a0
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DOI: https://doi.org/10.1038/183558a0
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