Preparation and Sectioning of Tissues embedded in ‘Araldite’ for Electron Microscope Examination

Abstract

OWING to the complete lack of polymerization damage and less-stringent hardening conditions, ‘Araldite’ is being increasingly used as an alternative to butyl methacrylate as an embedding medium in the preparation of tissue for study in the electron microscope. Unfortunately, it has one serious drawback of its own. Unless the consistency of the plastic falls within fairly narrow limits, serious wrinkling is found on sectioning which may make the specimens quite unsuitable for study.