Abstract
THE total, non-protein and protein sulphydryl contents of biological materials can be determined by using two different analytical procedures: one for the determination of total sulphydryl (protein sulphydryl + non-protein sulphydryl) values and the other for the determination of non-protein sulphydryl values. The protein sulphydryl values are obtained by subtracting the non-protein sulphydryl values from the protein sulphydryl values. Most of the available methods, however, are often complicated, time-consuming and more or less non-specific. In an earlier communication1 it was reported from this laboratory that, with a little modification, the amperometric method of Benesch, Lardy and Benesch2 can be used for the specific determination of the total sulphydryl content of biological materials. It has since been observed that the method can be adapted for the non-protein sulphydryl determinations in the same materials. Thus it is possible to use a simple, specific and rapid method for the determination of all the three sulphydryl fractions in biological materials.
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References
Bhattacharya, S. K., Biochem. J., 69, 43p (1958).
Benesch, R. E., Lardy, H. A., and Benesch, R., J. Biol. Chem., 216, 663 (1955).
Benesch, R. E., and Benesch, R., Arch. Biochem., 28, 43 (1950).
Benesch, R., and Benesch, R. E., Arch. Biochem., 19, 35 (1948).
Goldzieher, J. W., Rawls, W. B., and Goldzieher, M. A., J. Biol. Chem., 203, 519 (1953).
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BHATTACHARYA, S. Amperometric Determination of Sulphydryl Content of Blood and Tissues. Nature 183, 1327 (1959). https://doi.org/10.1038/1831327a0
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DOI: https://doi.org/10.1038/1831327a0
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