Abstract
PREVIOUS communications from this Laboratory1,2 have reported changes in the amino-acid content of synthetic medium M 150 during cultivation of a variety of freshly explanted tissues and have established a characteristic pattern of change with each tissue studied3. Cultivation of strain L (Earle) cells of mouse fibroblastic origin revealed a pattern of changes in the synthetic medium differing significantly from that of the freshly explanted tissues in regard to the metabolism of glutamine4. These studies have now been extended to a number of cell lines of malignant origin and appear to have established a consistent difference between the two types of cultures. All cultures of freshly explanted tissues so far examined accumulate glutamine in the culture medium, while all established cell lines studied utilize this amide from the surrounding fluid.
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References
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PASIEKA, A., MORGAN, J. Glutamine Metabolism of Normal and Malignant Cells cultivated in Synthetic Media. Nature 183, 1201–1202 (1959). https://doi.org/10.1038/1831201a0
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DOI: https://doi.org/10.1038/1831201a0
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