Abstract
SOME pure 3,6 anhydro-L-galactose was required as standard to determine the concentration of this sugar in several algal polysaccharides. Efforts to prepare it in a crystalline form were unsuccessful. Therefore the standard solution was made up from the 3,6 anhydro-L-galactosediethylmercaptal. Unfortunately the ethylmercaptan liberated on acid hydrolysis of the mercaptal interfered with colour formation in the anthrone and Seliwanoff tests. A similar interference was obtained with the diphenylamine reagent1. A method was developed to obtain from 3,6 anhydro-L-galactosediethylmercaptal, in quantitative yield, a solution of 3,6 anhydro-L-galactose free of ethylmercaptan. The object of this communication is to describe the method whereby sugar mercaptals can be analysed with colorimetric sugar reagents.
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YAPHE, W. Determination of Galactosediethylmercaptal and 3,6 Anhydrogalactosediethylmercaptal with the Anthrone and Seliwanoff Reagents. Nature 183, 44–45 (1959). https://doi.org/10.1038/183044b0
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DOI: https://doi.org/10.1038/183044b0
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