Abstract
Grassmann and Hübner1 have pointed out the possibility of applying their continuous paper electrophoresis to the detection of loosely bound molecular compounds. The example they gave was a pair of dyes, orange II and methylene blue. These substances were introduced continuously on to a hanging paper at two separate points. They ran down vertically due to gravity and at the same time migrated horizontally towards each other under the influence of the applied electrical field. It was anticipated that the procedure would be applicable not only to the molecular compounds of dyestuffs but also to antigen–antibody complex formation. However, simpler methods have been devised for the antigen–antibody reaction2–4.
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References
Grassmann, W., and Hübner, L., Naturwiss., 40, 272 (1953).
Oudin, J., C.R. Acad. Sci., Paris, 222, 115 (1946); 228, 1890 (1949). Bull. Soc. Chim. Biol., 29, 140 (1947).
Ouchterlony, Ö., Acta Path. Microbiol. Scand., 25, 186 (1948); 32, 230 (1953).
Grabar, P., and Williams, C. A., Biochim. Biophys. Acta, 10, 193 (1953); 17, 67 (1955).
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NAKAMURA, S., UETA, T. ‘Crossing’ Paper Electrophoresis for the Detection of Immune Reactions. Nature 182, 875 (1958). https://doi.org/10.1038/182875a0
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DOI: https://doi.org/10.1038/182875a0
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