Abstract
PREVIOUS studies on the osmotic properties of isolated nuclei indicated that ions or molecules of low molecular weight readily penetrate the nuclear membrane. Of the components tested, only protein and certain other substances of high molecular weight were found to exert an osmotic effect1,2. The process of isolation may have marked effects on the properties of the nucleus and its membrane. In the present studies, osmotic properties of the nucleus were studied by micro-injection of certain solutions into the cytoplasm. Relatively small, unpigmented oocytes of Rana pipiens were used. Photographic records were obtained and all measurements were made from these. No harmful effects were observed within the cells following injection.
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HARDING, C., FELDHERR, C. Semipermeability of the Nuclear Membrane. Nature 182, 676–677 (1958). https://doi.org/10.1038/182676a0
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DOI: https://doi.org/10.1038/182676a0
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