Abstract
EFFORTS to detect extracted iodized compounds directly on chromatograms have been made by various authors while following the metabolism of thyroid hormones. Nevertheless, because of low sensitivity of the agents currently employed (ninhydrin, diazotized sulphanilic acid) this has proved fruitless. By modifying the original Sandell–Kolthoff reaction for iodine estimation, Bowden et al. 1 succeeded in detecting 0.1γ of thyroxine. The detection of thyroxine following chromatography of serum required the use of large amounts of the biological material2. Moreover, careful purification of the extract for chromatography was laborious and time-consuming. Efforts made so far1,3 have likewise failed to bring about the desired results because the colour spots are of short duration.
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References
Bowden, C. H., Maclagan, N. F., and Wilkinson, J. H., Biochem. J., 59, 93 (1955).
Fletcher, K., and Stanley, P. G., Nature, 175, 730 (1955).
Dragúnová, I., and Langer, P., Nature, 178, 537 (1956).
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ŠTOLC, V. Chromatographic Detection of Minute Quantities of Thyroxine in Serum. Nature 182, 52 (1958). https://doi.org/10.1038/182052a0
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DOI: https://doi.org/10.1038/182052a0
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