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Incorporation of Disodium p-Nitrophenylphosphate in Solid Medium for the Detection of Phosphatase Production by Micrococcus pyogenes var. aureus


THE production of phosphatase by Micrococcus pyogenes var. aureus has been demonstrated using a solid medium incorporating phenolphthalein phosphate1. The presence of phosphatase has also been correlated with coagulase production2, which in turn has been shown to be linked with exotoxin production3. Therefore it is assumed that all exotoxinproducing staphylococci are coagulase- and phosphatase-positive. The quantitative study of the phosphatase activity of Micrococcus pyogenes var. aureus 4 has shown that the production of phosphatase can be used as an index to toxin production.

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  1. 1

    Barber, Mary, Brookbank, B. W. L., and Kuper, S. W. A., J. Path. Bact., 63, 57 (1951).

  2. 2

    Barber, Mary, and Kuper, S. W. A., J. Path. Bact., 63, 65 (1951).

  3. 3

    Walder, Winifred O., Lab. Practice, 2, 419 (1953).

  4. 4

    Barnes, Ervine, H., and Morris, James F., J. Bact., 73, 100 (1957).

  5. 5

    Bessey, O. A., Lowry, O. A., and Brock, M. J., J. Biol. Chem., 164, 321 (1946).

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