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Retention of Sodium-22 in Rat Bone

Abstract

THREE groups of male rats, of about 50, 150 and 300 gm. body-weight respectively, were placed on a low sodium diet, and 5 µc. sodium-22 chloride per kgm. body-weight injected intraperitoneally on day 1. On days 3 and 5 a further injection of 2 µc. per kgm. was given, and on day 7 all were replaced on the stock diet (0.5 per cent sodium). Six female rats each weighing 250 gm. were mated on day 1, and the diet and sodium-22 administered as above; three were replaced on the stock diet on day 14 and the others maintained on the low sodium intake until term. Approximately each 10 days, commencing on day 11, one male from each group was bled from the tail to obtain a plasma sample; humeri and femora were then removed and scraped as clean as possible and weighed. The four bones from each animal were placed in 0.05 M silver nitrate in 5 N nitric acid, and after standing in the cold for some days the mixture was heated at 100° C. to effect complete solution of organic matter. This method was designed to avoid formation of volatile organic compounds containing chlorine1,2.

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KELLERMAN, G. Retention of Sodium-22 in Rat Bone. Nature 181, 914–915 (1958). https://doi.org/10.1038/181914a0

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