Effect of Testosterone Propionate upon the Incorporation of Labelled Glycine into Homogenates of Mouse Kidney

Abstract

IT was shown recently¹ that the rate of incorporation of glycine-l-¹⁴C into kidney slice protein of A/Jax mice treated with testosterone propionate was significantly higher than into slices from untreated animals. The maximum increase (35–80 per cent) appeared 48 hr. after beginning daily injections of 1 mgm. testosterone propionate, after which the difference between the treated and control series gradually disappeared. A study of some of the characteristics of this response revealed that the difference in incorporation-rates was greatest at low glycine concentrations, and diminished when the glycine concentration of the incubation medium was raised to 4–8 mM. It was postulated that under these conditions the effect of this steroid was to facilitate the intracellular accumulation of glycine from a relatively glycine-deficient environment.