Sudanophil Inclusions in Ameloblasts, Odontoblasts and Cells of the Oral Epithelium

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Abstract

DURING studies of the lipid inclusions in cells of teeth and of the oral cavity of the rat, Baker's sudan black B method1 was employed. The oral soft tissues were fixed in formalin–calcium solution, postchromed, embedded in gelatin, and cut on the freezing microtome. The enamel organ and pulp were dissected from the upper incisor tooth and similarly treated. Equally good results could be obtained from teeth if the entire tissue was decalcified with ethylenediamine tetraacetic acid (0.5 M) at pH 7.4, or with 10 per cent acetic acid, after fixation and before post-chroming. Beams and King2 had investigated the Golgi bodies of dental tissues using Nassanow's method3, and so this procedure was also used. Pyridine extraction of the tissues using Baker's method4 was made in some cases. The results of the two methods agreed well on oral mucosa and that of the tongue.

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References

  1. 1

    Baker, J. R., Quart. J. Micro. Sci., 90, 293 (1949).

  2. 2

    Beams, H. W., and King, R. L., Anat. Rec., 57, 29 (1933).

  3. 3

    Nassanow, D. N., Arch. mikr. Anat., 97, 136 (1923).

  4. 4

    Baker, J. R., Quart. J. Micro. Sci., 87, 458 (1946).

  5. 5

    Baker, J. R., Symp. Soc. Exp. Biol., “Mitochondria and other Cytoplasmic Inclusions”, 10 (1957).

  6. 6

    Symons, J. Dent. Res., 36, 805 (1957).

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IRVING, J. Sudanophil Inclusions in Ameloblasts, Odontoblasts and Cells of the Oral Epithelium. Nature 181, 569–570 (1958) doi:10.1038/181569a0

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