Demonstration of Some ‘Masked’ Lipids in the Oocytes of Chrotogonus trachypterus with Sudan Black B and Phenol ‘Unmasking’ Techniques

Article metrics

Abstract

SUDAN black B, in spite of being the most specific and vigorous dye for the demonstration of lipids in tissue sections1,2, has its limitations3,4. The cell inclusion believed to be lipid may not be coloured with sudan black B, because: (1) some lipids (like phospholipids) may escape from the tissue into the fixative by the process of emulsification2,3; (2) the lipids present have a high melting point and thus remain ‘solid’ in tissue sections3; or (3) the lipids present are ‘masked’ by proteins or other substances.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Pearse, A. G. E., “Histochemistry” (J. and A. Churchill, Ltd., London, 1954).

  2. 2

    Baker, J. R., Quart. J. Micro. Sci., 87, 44 (1946); 90, 293 (1949); 97, 161 (1957).

  3. 3

    Cain, A. J., Biol. Rev., 25, 73 (1950).

  4. 4

    Bradbury, S., Quart. J. Micro. Sci., 97, 499 (1956).

  5. 5

    Nath, V., et al. (in the press).

  6. 6

    Gomori, G., “Microscopic Histochemistry” (University Press, Chicago, 1952).

  7. 7

    Lovern, J. A., “The Chemistry of Lipids of Biochemical Significance” (Methuen and Co., Ltd., London, 1955).

  8. 8

    Casselman, W. G. B., and Baker, J. R., Quart. J. Micro. Sci., 96, 49 (1955).

  9. 9

    Ciaccio, G., Boll. Soc. Biol. Speri., 1, 47 (1926).

  10. 10

    Chiffelle, T. L., and Putt, F. A., Stain Tech., 26, 51 (1951).

  11. 11

    Mazia, D., Brewer, P., and Alfert, M., Biol. Bull., 104, 57 (1953).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

GUPTA, B. Demonstration of Some ‘Masked’ Lipids in the Oocytes of Chrotogonus trachypterus with Sudan Black B and Phenol ‘Unmasking’ Techniques. Nature 181, 555 (1958) doi:10.1038/181555a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.