Letter | Published:

Fibrinolysis Autographs

Abstract

Permin and Astrup used a plate method of demonstrating fibrinolytic activity1,2. This technique can be applied histologically. A thin layer of fibrin is made on a microslide by adding a drop or two of fibrinogen to a film of thrombin on the glass. When the fibrin layer has formed, a section of fresh or alcohol-fixed tissue cut on the freezing microtome is applied to its surface and the preparation incubated at 37° C. After 30–60 min. patches of digestion of the fibrin appear in relation to structures possessing fibrinolytic activity. These patches appear as clear zones when the preparations are stained with hæmalum and eosin (Fig. 1), and can be related to the structures in the section.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Permin, P. M., Nature, 160, 571 (1947).

  2. 2

    Astrup, T., in “Blood Coagulation and Allied Problems”. Transactions of the First Conference of the Josiah Macy, Jr. Foundation, New York (1948).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.