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Method of isolating the β1-Metal-combining Globulin from Human Blood Plasma


Surgenor, Koechlin and Strong1,2 have described the isolation of the β1-metal-combining globulin (siderophilin, transferrin) from Cohn's plasma fraction IV–4 by alcohol fractionation. The crystallization, described by Koechlin2, could not easily be reproduced by other investigators. Improved conditions for the purification and the crystallization starting from fraction IV–7 have recently been published by Inman3. Already the preparation of fraction IV–7 requires many steps and much time, which may be one of the reasons why only very few clinical experiments with this iron-transmitting globulin have been reported.

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  1. 1

    Surgenor, D. M., Koechlin, B. A., and Strong, L. E., J. Clin. Invest., 28, 73 (1949).

  2. 2

    Koechlin, B. A., J. Amer. Chem. Soc., 74, 2649 (1952).

  3. 3

    Inman, J. K., 10th Conf. on Plasma Proteins and Cellular Elements of the Blood, 1956 (Protein Foundation, Cambridge, Mass.).

  4. 4

    Hořejši, J., and Smetana, R., Acta Med. Scand., 155, 65 (1956).

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