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Occurrence of Foldings of Plasma Membrane (β-Cytomembrane) in Cells of Pig's Carpal Organ as revealed by Electron Microscopy

Abstract

THE carpal organ of the pig is a conglomerated sweat gland, and histologically it is known to be similar to the usual small sweat gland (eccrine gland)1. This organ is useful for investigating the fine structure of sweat glands with the electron microscope, since the conglomerated body of the gland is easily seen with the naked eye and most of the glandular tissue is found within the small field of the electron microscope. The tissue was fixed in 1 per cent osmic acid and buffered at pH 7.4 with acetate–veronal or phosphate buffer, washed for a short time and then dehydrated with ethanol in a graded series of concentrations. The specimens were embedded in a mixture of n-butyl and methyl methacrylate (7 : 3), and were cut into sections less than 0.05µ thick with a thermal expansion microtome (JUM-4) fitted with a glass knife. Embedded sections were examined in a JEM-3 electron microscope.

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References

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    Kitamura, T., Arch. Hist. Jap. (in the press).

  2. 2

    Ito, T., Folia Anat. Jap., 22, 274 (1943).

  3. 3

    Montagna, W., “The Structure and Function of Skin” (New York, 1956).

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    Sjöstrand, F. S., “Physical Techniques in Biological Research”, 3, (New York, 1956).

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    Maxwell, D. S., and Pease, D. C., J. Biophys. Biochem. Cytol., 2, 467 (1956).

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    Palade, G. E., J. Biophys. Biochem. Cytol., 2, Supp., 85 (1956).

  7. 7

    Dalton, A. J., and Felix, M. D., J. Biophys. Biochem. Cytol., 2, Supp., 79 (1956).

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