Letter | Published:

Electrophoretic Removal of Protein from Hyaluronic Acid


EVIDENCE has been presented1–3 to show that the hyaluronic acid of ox synovial fluid exists as a complex with a specific protein4, the latter constituting 25–30 per cent of the total complex. This evidence is, in brief, that hyaluronic acid isolated from synovial fluid by a sufficiently mild means (differential filtration) cannot be obtained free from this amount of protein, and that removal of the protein (as by proteolytic action) from the material so isolated profoundly alters the physicochemical properties of the remaining hyaluronic acid.

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    Smithies, O., D.Phil. thesis, Oxford (1951).

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