Isolation of a Soil Coccus capable of utilizing ‘Tween 80’ as a Sole Source of Carbon

Article metrics


IT is interesting that some organisms can utilize as their source of carbon a synthetic substance such as ‘Tween 80’ (polyoxyethylene sorbitanmono-oleate). My collaborators and I1 have proved that tubercle bacilli can utilize ‘Tween 80’ as a sole source of carbon, and Yamane et al. 2 then devised a simple and practicable ‘Tween’-agar medium for tubercle bacilli containing ‘Tween 80’ as a main source of carbon. I wished to investigate3 the details of the mechanisms of ‘Tween’ metabolism, but tubercle bacilli are not suitable for this purpose because their growth is so slow, their endogenous substrates are too abundant4, and experiments with the bacilli can be dangerous. I therefore decided to try to isolate bacteria more suitable for the purpose from the soil.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Minami, K., Yamane, I., and Yasui, T., Fukushima J. Med. Sci., Japan, 1, 95 (1954). Yamane, I., Minami, K., and Yasui, T., C.R. Soc. Biol., Paris, 148, 769 (1954).

  2. 2

    Yamane, I., Hyodo, S., Honda, T., and Kadoya, K., Fukushima J. Med. Sci., Japan, 2, 35 (1955).

  3. 3

    Minami, K., and Yamane, I., Symposium on Enzyme Chem., Japan, 10, 93 (1954).

  4. 4

    Edson, N. L., Bact. Rev., 15, 147 (1951).

  5. 5

    Breed, R. S., Murray, E. G. D., and Hitchens, A. P., “Bergey's Manual of Determinative Bacteriology”, sixth edit. (Williams and Wilkins Co., Baltimore, 1948).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

MINAMI, K. Isolation of a Soil Coccus capable of utilizing ‘Tween 80’ as a Sole Source of Carbon. Nature 181, 430–431 (1958) doi:10.1038/181430b0

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.