Occurrence of Rhodanese in a Species of Thiobacillus

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THE enzyme rhodanese which catalyses the reaction: has been shown to be present in Thiobacillus thiocyanoxidans. Cells of the organism, grown in thiosulphate medium with forced aeration and 2–5 per cent of carbon dioxide added1, were harvested in a de Laval centrifugal separator, washed and suspended in M/15 phosphate buffer, pH 7.0.

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  1. 1

    Vishniac, W., J. Bact., 64, 363 (1952).

  2. 2

    Sorbo, B. H., Acta Chem. Scand., 7, 1129 (1953).

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McCHESNEY, C. Occurrence of Rhodanese in a Species of Thiobacillus . Nature 181, 347–348 (1958) doi:10.1038/181347a0

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