The Reflectance Spectrum of Acetone-2,4-dinitrophenylhydrazone

Abstract

THE advantages afforded by the use of spectral reflectance for in situ studies of biochemical systems have been pointed out recently¹. Characterizing reflectance spectra of the hæm pigments in tuna meat were obtained which were identical with the absorption curves found from transmission measurements made on the same pigments in solution. Although the extension of this technique for the purpose of obtaining useful spectra of organic compounds directly on filter paper would appear both logical and desirable, published studies on this aspect of spectral reflectance are meagre^2,3. The studies, primarily concerned with the visible region, apparently have overlooked the equally important ultra-violet portion of the spectrum.