Antioxidant Determinations by the Use of a Stable Free Radical

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Abstract

METHODS for measuring antioxidants and appraising antioxidant activity appear to be of two general types. If the chemical nature of the antioxidant is known, one may strive for a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups. Alternatively one may observe the inhibition of some natural oxidative process such as the β-oxidation of fats, as a function of the added antioxidant.

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References

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    Goldschmidt, S., and Renn, K., Chem. Ber., 55, 628 (1922). Poirier, R. H., Kahler, E. J., and Benington, F., J. Org. Chem., 17, 1437 (1952). Braude, E. A., Brook, A. G., and Linstead, R. P., J. Chem. Soc., 3574 (1954).

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    Blois, M. S., Biochim. Biophys. Acta, 18, 165 (1955).

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BLOIS, M. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181, 1199–1200 (1958) doi:10.1038/1811199a0

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