Letter | Published:

Enzymic Synthesis of Nitrogen-containing Disaccharides by α-Galactosyl Transfer

Abstract

SEROLOGICAL and enzymic inhibition tests with simple sugars and oligosaccharides of known structure have in recent years given valuable indications about the sugars which are most important for specificity in the complex mucopolysaccharide materials possessing blood-group activity1. Di-and tri-saccharides of known structure which are composed of the sugars present in the blood-group substances are required in order to extend these observations; the enzymic syntheses of two such disaccharides which have not previously been described, α-D-galactosyl-(1→6)-N-acetyl-D-glucosamine (1) and α-D-galactosyl-(1→6) - N -acetyl-D-gal-actosamine (2), are reported here.

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References

  1. 1

    Watkins, W. M., and Morgan, W. T. J., Nature, 169, 825 (1952). Morgan, W. T. J., and Watkins, W. M., Brit. J. Exp. Path., 34, 94 (1953). Kabat, E. A., and Leskowitz, S., J. Amer. Chem. Soc., 77, 5159 (1955). Watkins, W. M., and Morgan, W. T. J., Nature, 175, 676 (1955); 178, 1289 (1956). Côté, R., and Morgan, W. T. J., ibid., 178, 1171 (1956).

  2. 2

    Zillikin, F., Smith, P. N., Rose, C. S., György, P., J. Biol. Chem., 208, 299 (1954). Alessandrini, A., Schmidt, E., Zilliken, F., and György, P., J. Biol. Chem., 220, 71 (1956).

  3. 3

    Wallenfels, K., Ann. Chem., 579, 113 (1952). Kuhn, R., Baer, H. H., and Gauhe, A., Chem. Ber., 88, 1713 (1955).

  4. 4

    Watkins, W. M., Biochem. J., 54, xxxiii (1953), and unpublished observations.

  5. 5

    Kuhn, R., Baer, H. H., and Gauhe, A., Chem. Ber., 88, 1713 (1955).

  6. 6

    Kuhn, R., Baer, H. H., and Gauhe, A., Chem. Ber., 87, 1553 (1954).

  7. 7

    Zilliken, F., Smith, P. N., Tomarelli, R. M., and György, P., Arch. Biochem. Biophys., 54, 398 (1955).

  8. 8

    Bacon, J. S. D., and Edelman, J., Biochem. J., 48, 114 (1951).

  9. 9

    Partridge, S. M., and Westall, R. G., Biochem. J., 42, 238 (1948).

  10. 10

    Jeanloz, R. W., and Trémège, M., Fed. Proc., 15, 282 (1954).

  11. 11

    Kuhn, R., Gauhe, A., and Baer, H. H., Chem. Ber., 87, 1138 (1954).

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