Letter | Published:

Isoleucine and Allo-isoleucine

  • An Erratum to this article was published on 08 March 1958

Abstract

DURING our investigations on amino-acid analysis on ion exchange resins using Moore and Stein's method1, it was seen that in a synthetic mixture of 13 amino-acids (Hoffmann-LaRoche, mostly in the l-configuration) the ‘methionine’ recovery was always too high (120–150 per cent of the theoretical amount) and the ‘isoleucine’ recovery always too low (65–70 per cent of the theoretical amount).

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References

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    Moore, S., and Stein, W. H., J. Biol. Chem., 192, 663 (1951).

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    Shulgin, A. T., Lien, O. G., Gal, E. M., and Greenberg, D. M., J. Amer. Chem. Soc., 74, 2427 (1952).

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    Witkop, B., and Beiler, Th., J. Amer. Chem. Soc., 78, 2882 (1956).

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    Hamilton, P. B., and Anderson, R. A., J. Biol. Chem., 213, 249 (1955).

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    Moore, S., and Stein, W. H., J. Biol. Chem., 176, 367 (1948).

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    Schram, E., Dustin, J. P., Moore, S., and Bigwood, E. J., Anal. Chem. Acta, 9, 149 (1953).

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