Letter | Published:

Detection of α-Ketoglutaric Acid and Other Keto-Acids on Paper Chromatograms with Ninhydrin


NUMEROUS paper chromatographic methods exist for the detection of keto-acids as their 2,4-dinitro-phenylhydrazone, semicarbazone, and quinoxaline derivatives or as the free acids1. By the ninhydrin spray test for amino-acids on paper chromatograms, it is now possible to detect as little as 1 µgm. per sq. cm. of α-ketoglutaric acid on chromatograms of plant extracts. The procedure described here thus permits simultaneous detection of the amino-acids on the same chromatogram.

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  1. 1

    Ranson, S. L., “Modern Methods of Plant Analysis”, 2, edit by Paech, K., and Tracey, M. V., 539 (Springer-Verlag, Berlin, 1955).

  2. 2

    Benson, A. A., Bassham, J. A., Calvin, M., Goodale, T. C., Haas, V. A., and Stepka, W., J. Amer. Chem. Soc., 72, 1710 (1950).

  3. 3

    Steward, F. C., and Pollard, J. K., “Ann. Rev. Plant Physiol.”, 8, 65 (1957); see p. 69.

  4. 4

    Ven Horst, H., Jurkovich, V., and Carstens, Y., Anal. Chem., 29, 788 (1957).

  5. 5

    Rabson, R., Ph.D. thesis, Cornell University (Sept. 1956).

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