Abstract
NUMEROUS paper chromatographic methods exist for the detection of keto-acids as their 2,4-dinitro-phenylhydrazone, semicarbazone, and quinoxaline derivatives or as the free acids1. By the ninhydrin spray test for amino-acids on paper chromatograms, it is now possible to detect as little as 1 µgm. per sq. cm. of α-ketoglutaric acid on chromatograms of plant extracts. The procedure described here thus permits simultaneous detection of the amino-acids on the same chromatogram.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ranson, S. L., “Modern Methods of Plant Analysis”, 2, edit by Paech, K., and Tracey, M. V., 539 (Springer-Verlag, Berlin, 1955).
Benson, A. A., Bassham, J. A., Calvin, M., Goodale, T. C., Haas, V. A., and Stepka, W., J. Amer. Chem. Soc., 72, 1710 (1950).
Steward, F. C., and Pollard, J. K., “Ann. Rev. Plant Physiol.”, 8, 65 (1957); see p. 69.
Ven Horst, H., Jurkovich, V., and Carstens, Y., Anal. Chem., 29, 788 (1957).
Rabson, R., Ph.D. thesis, Cornell University (Sept. 1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
RABSON, R., TOLBERT, N. Detection of α-Ketoglutaric Acid and Other Keto-Acids on Paper Chromatograms with Ninhydrin. Nature 181, 50–51 (1958). https://doi.org/10.1038/181050a0
Issue Date:
DOI: https://doi.org/10.1038/181050a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
