Letter | Published:

A Highly Specific Growth-inhibitory Factor produced by Certain Acetobacter Species

Nature volume 180, pages 811812 (19 October 1957) | Download Citation

Subjects

Abstract

RECENT studies1 with a cellulose-producing culture of Acetobacter acetigenum NCIB 8132 revealed that this culture contained mutant organisms which were unable to produce extracellular cellulose (celluloseless mutants). Their presence in the wild-type culture was indicated by a mass-plating technique in which a large inoculum from a cellulose-producing culture was spread on the surface of glucose (3 per cent, w./v.), ‘Difco’ yeast-extract (0.5 per cent, w./v.) (GYE) medium (pH 7.0) containing agar (2 per cent, w./v.) ; mutant organisms showed a different colony type and a faster growth-rate than wild-type organisms. In GYE liquid medium parent organisms produced a cellulose pellicle, the medium under the pellicle remaining virtually clear. In contrast, mutant organisms grew diffusely in liquid medium and produced a moderate degree of turbidity. Despite the fact that mutant organisms could be detected in wild-type cultures by the mass-plating procedure, all our attempts to isolate mutants from cellulose-producing cultures by the indirect-select ion method of Lederberg and Lederberg2 have been unsuccessful. The present work was undertaken in an attempt to clarify this contradictory behaviour.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , and , J. Gen. Microbiol. (in the press).

  2. 2.

    , and , J. Bact., 63, 399 (1952).

Download references

Author information

Affiliations

  1. College of Science and Technology, University of Manchester.

    • ROBERT STEEL
    •  & THOMAS K. WALKER

Authors

  1. Search for ROBERT STEEL in:

  2. Search for THOMAS K. WALKER in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/180811a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.