Letter | Published:

Bacterial Protoplasts from Bacillus Species by the Action of Autolytic Enzymes

Nature volume 180, pages 759760 (12 October 1957) | Download Citation

Subjects

Abstract

WHEN certain bacteria are suspended in sucrose solution of suitable concentration and incubated with lysozyme, the rigid cell-walls are dissolved away, leaving relatively stable spherical protoplasts1. Lytic enzymes which dissolve the isolated cell-walls of vegetative Bacillus cereus have been found in extracts of mechanically disintegrated resting spores2 and partial autolysates of sporulating cells3 of this organism. When heat-treated, intact vegetative cells were treated with preparations of these enzymes, the walls were dissolved, leaving the coagulated cell-contents apparently unchanged. It has now been found that when viable organisms are suspended in sucrose solution and treated with these enzymes, relatively stable protoplasts are obtained in good yield.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    Symp. Soc. Gen. Microbiol. “Bacterial Anatomy”, edit. E. T. C. Spooner and B. A. D. Stocker, p. 111, (Camb. Univ. Press, 1956).

  2. 2.

    , and , J. Gen. Microbiol., 16, 236 (1957).

  3. 3.

    , and , J. Gen. Microbiol., 17 (in the press).

Download references

Author information

Affiliations

  1. Ministry of Supply, Microbiological Research Establishment, Porton, Wilts.

    • F. A. DARK
    •  & R. E. STRANGE

Authors

  1. Search for F. A. DARK in:

  2. Search for R. E. STRANGE in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/180759a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.