IN the course of an investigation of the changes in composition of the digesta along the alimentary tract of the sheep1, it was noted that there was a considerable addition of nitrogen to the chyme in the most proximal quarter of the small intestine. The nitrogen content in the remaining quarters fell off distally until the abomasal level was attained in the fourth quarter. It was noted from in vivo studies on fistulated animals, and in those with exteriorized loops of the small intestine within the first two feet or so of the pylorus, that this addition of nitrogen did not occur to the same extent as in the shot sheep. Lignin was used as a reference substance to assess these changes.
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Boyne, A. W., Campbell, Rosa M., Davidson, J., and Cuthbertson, D. P., Brit. J. Nutr., 10, 325 (1956)
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BADAWY, A., CAMPBELL, R., CUTHBERTSON, D. et al. Changes in the Intestinal Mucosa of the Sheep following Death by Humane Killer. Nature 180, 756–757 (1957). https://doi.org/10.1038/180756b0
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