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Fractionation of Isolated Lucerne and other Triterpenoidal Saponins

Naturevolume 180pages12971298 (1957) | Download Citation



THE constituent saponins of bulk triterpenoidal saponins of lucerne and certain other plant materials did not separate satisfactorily on paper chromatograms with various solvents used for other saponins1. It has been found that modified solvent systems or multiple development give the most promising results2. Ascending paper chromatography was more convenient ; long running times with descending paper chromatography produced streaking with many saponins. Horizontal paper electrophoresis with weak borate buffer was also used successfully.

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  1. 1

    Belič, I., Nature, 178, 538 (1956). Dutta, N. L., ibid., 175, 85 (1955). Fontan-Candela, J. L., An. Real. Soc. Exp. Fis. Quim., 57, B, 432 (1955).

  2. 2

    Coulson, C. B., Biochem. J., 67, 10P (1957) and in the press.

  3. 3

    Paseshnichenko, V. A., and Guseva, A. R., Biochimija, 21, 585 (1956).

  4. 4

    Walter, E. D., Van Atta, G. R., Thompson, C. R., and Maclay, W. D., J. Amer. Chem. Soc., 76, 2271 (1954).

  5. 5

    Lindahl, I. L., Davis, R. E., Dougherty, R. W., Thompson, C. R., Wilson, R. H., and others, “Alfalfa saponins” (United States Department of Agriculture, Technical Bulletin No. 1161 (April 1957)).

  6. 6

    Johns, A. T., Vet. Rev. and Annotations, 2, 107 (1956).

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  1. Department of Agricultural Chemistry, University College of North Wales, Bangor

    • C. B. COULSON


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